Human Embryonic Stem Cell Culture in Microfluidic Channels
نویسندگان
چکیده
We demonstrate the first successful culture of human embryonic stem cells in microscale polydimethylsiloxane channels. The effects of two different feeder layers (irradiated mouse embryonic fibroblasts and a gel matrix (Matrigel, BD Biosciences)) on cell growth and morphology are investigated. Cell growth over a one-week period follows the normal pattern of attachment, colony expansion, and eventual formation of three-dimensional colony structures.
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